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Natural transformation of the filamentous cyanobacterium

nbsp 0183 32 The cell suspension was homogenized using an Ultraturrax Silent Crusher M Heidolph Schwabach Germany with the dispersion tool 18F at 10 000 rpm for 3 min The cell suspension was centrifuged at 6000 g and 4 C for 15 min After each centrifugation...

Effects of the chemical and physical reaction conditions on

nbsp 0183 32 Then the starch solution was homogenized Heidolph Silent Crusher M Germany for 5 min at ambient temperature to completely dissolve the starch solution Stearic acid 5 10 or 25 mg was dissolved in 100 mL of 95 ethanol and the solution was added dropwise to the starch solution at various addition rates 2 4 8 or 16 mL/min and at various temperatures 70 80 or 90 176 C...

Fabrication of β

nbsp 0183 32 In order to reduce the droplet size of produced coarse emulsions a high speed homogenizer Heidolph Silent Crusher M Homogenizer Germany at 12 000 rpm 6 min was applied 2 4 Emulsion characterization...

Full article Genetically modified Pichia pastoris a powerful

nbsp 0183 32 v/v Triton X100 10 mM Tris pH = 8 and subjected to a mechanical cell rupture Silent Crusher Heidolph Germany Afterward the lysate was clarified three times with Tris/HCl buffer 1 5 M pH 8 3 containing 1 w/v SDS Nanometals were purified...

Full article Ganoderic acid loaded nano

nbsp 0183 32 The GA-loaded NLCs were developed by hot ME technique Heidolph Silent Crusher M Heidolph Germany In brief GA and lipid mixtures Cap MCM C10 Capmul PG8 were melted with addition of ethanol solution of PL-90G and Tween 80 with...

Formulation of topical NLCs to target macrophages for

nbsp 0183 32 Aqueous phase consisted of tween 80 dissolved in distilled water maintained at same temperature as organic phase i e at 65 C Organic phase was added at once to the aqueous phase while stirring at 26000 rpm using homogenizer Heidolph silent crusher M...

Often the Subtle Symphony of often the Winery

nbsp 0183 32 Anyone who has proved helpful in a winery regarding any length of period learns there is one sound more than another that will is instantly pump-stoppingly drop-what-you-are-doing alarming the sound involving liquids falling on often the floor That has a a number...

Growtopia in 2020

nbsp 0183 32 since within 2 weeks we have reached 365/365 of 2019 times has passed by so fast let s discuss what Growtopia in 2020 by their update with ubisoft canada and abu dhabi i know it s still long to discuss but it s good to chitchat with us as community to think their...

Cytotoxic anti

nbsp 0183 32 Well-grown mycelial mass was homogenized using a Heidolph Silent Crusher then 30 other flasks containing the same medium composition were inoculated using 2 mL of homogenized culture The flasks were incubated at 23 176 C under constant shaking at 140 rpm on a rotary shaker...

Expression and functional analysis of the propamocarb

nbsp 0183 32 A mixture of the cucumber leaf sample 12 5 g with 25 mL of acetonitrile was homogenized with a high-speed homogenizer Heidolph Silent Crusher-M 174 for 2 min at 15 000 215 g and incubated at room temperature for 0 5 h...

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